FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071641
Sequences flagged as poor quality0
Sequence length35-251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTGG18550.052.4695247
GAGTAGC11850.051.6036074
AGTAGCT17200.051.1955835
CGAGTAG4550.051.0705643
TACTCGG5050.050.857653
GCTGGGA36450.050.6482059
GTAGCTG17200.048.708466
CCGAGTA4800.045.6017272
GGAGGCT24050.043.7187779
TCGGGAG6300.043.0235376
AGTGCTG22950.042.4100076
TTGGGAG20850.041.363376
TGCTGGG28250.040.2484478
AAAGTGC23400.039.7215464
CCCAAAG30450.038.3383981
GTGCTGG25800.038.2027177
CTACTCG6750.037.8325462
GCTACTC13200.037.771131
AAGTGCT24450.037.5155075
CCAAAGT28000.036.9156882
Produced by FastQC (version 0.11.5)