FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1092323
Sequences flagged as poor quality0
Sequence length35-351
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA7500.059.9699752
GCTGGGA57650.059.8139349
TACTCGG7200.053.21413
CCCGAGT10500.052.354741
CGAGTAG7750.051.5870743
GGAGGCT40300.050.0163159
TAGCTGG29600.049.5247547
AGTAGCT28450.048.5989845
GTAGCTG29700.047.114466
CCCAAAG53600.046.618451
GAGTAGC17050.045.920314
AGTGCTG44750.044.6703726
CCAAAGT49900.044.399952
CTACTCG7700.043.268382
AAAGTGC43850.042.9279564
GTGCTGG46400.042.3638467
AAGTGCT47550.041.3392755
CGGAGGT10100.041.2334827
CAAAGTG49050.040.0750773
TCGGGAG10900.039.735466
Produced by FastQC (version 0.11.5)