FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4587624
Sequences flagged as poor quality0
Sequence length100-150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG23050.062.9153822
TACTCGG23600.062.471243
GCTACTC55950.059.3194581
CTCGGGA27950.055.9906545
CGAGTAG15350.055.5025633
CCGAGTA16050.055.3425032
CGTCGCG551.1996364E-554.9102449
GGAGGCT122450.054.6053129
GCTGGGA146000.054.1956569
TCGGGAG30000.053.1548546
ACTCGGG29000.052.9222154
CCCGAGT18600.049.728281
TGCTGGG117150.048.6200568
CGCGATA504.943594E-448.3399852
GTGCTGG109400.047.9792257
CCCAAAG134450.047.6167831
AAAGTGC117400.045.909134
GAGTAGC35550.045.380994
AGTGCTG117650.045.3345226
GTAGCTG60000.044.1950386
Produced by FastQC (version 0.11.5)