FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3820067
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG21950.065.60473
CTACTCG22150.065.023392
CGAGTAG17950.063.510773
CCGAGTA19400.060.629852
GCTGGGA170250.057.822939
GAGTAGC39100.057.6961144
CCCGAGT21650.056.914431
GCTACTC52200.056.8830531
GTAGCTG67850.053.4984256
CGTCGAC650.00658188453.23283115-119
TCGGGAG30200.053.0444346
AGTAGCT67500.052.4425355
CCCAAAG133900.051.5332761
ACTCGGG29000.051.1015934
AAAGTGC117100.050.9802134
CTCGGGA29850.050.8524255
GGAGGCT127000.050.6844379
AGTGCTG120750.050.1845136
GTGCTGG123400.049.7874957
TAGCTGG76150.049.479497
Produced by FastQC (version 0.11.5)