FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3820067
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG15600.077.6929863
CCGAGTA17150.073.4719542
GCTACTC62800.063.648631
TACTCGG27550.063.594023
CTACTCG28050.062.8898962
CCCGAGT19750.062.600041
GAGTAGC43400.060.413694
GCTGGGA176400.058.9283459
GTAGCTG67400.057.57966
AGTAGCT69050.057.0865755
TCGGGAG33750.056.5168046
ACTCGGG32000.056.4365164
GGAGGCT147900.054.7912839
AAAGTGC120650.054.4540824
CTCGGGA33700.054.1222725
CCCAAAG139150.053.612051
AGTGCTG128150.053.0785066
CCAAAGT131400.052.650242
TGCTGGG133450.052.5436448
GTGCTGG127550.052.294297
Produced by FastQC (version 0.11.5)