FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4392657
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAG2800.066.343711
CTACTCG19600.055.8666572
TACTCGG20950.055.6819343
CGAGTAG14800.053.3551033
CCGAGTA15950.051.0194632
CGCGACT855.8698788E-849.348641
GCTACTC49900.048.875361
GCTGGGA157850.048.1620949
TCGGGAG28200.046.4556966
CCCGAGT19700.045.322741
CTCGGGA26750.044.7259985
ACTCGGG27700.044.0559164
GGAGGCT118800.043.9040379
GAGTAGC38300.043.7333874
CCCAAAG129850.041.810211
TGCTGGG122250.040.85478
AGTGCTG117200.040.7814946
GTGCTGG116450.040.4277157
AAAGTGC116850.040.2902374
GTAGCTG68700.039.7056276
Produced by FastQC (version 0.11.5)