FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4392657
Sequences flagged as poor quality0
Sequence length100-150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG24050.064.676533
CTACTCG24600.063.719072
GCTACTC58950.053.804551
CCGAGTA14250.052.9004022
TCGGGAG30800.052.2278446
GCTGGGA160750.051.633219
CGAGTAG14350.050.028533
GGAGGCT129600.049.278439
CTCGGGA32050.049.0909355
ACTCGGG31400.048.964584
GTGCTGG114550.046.461217
CCCAAAG129850.045.0276761
GAGTAGC37300.044.9084134
TGCTGGG126400.044.8972438
AGTAGCT66100.044.1663935
AAAGTGC115950.044.010554
CCCGAGT18700.043.843571
AGTGCTG120500.043.423176
GTAGCTG67550.042.5814326
AAGTGCT123900.041.8621375
Produced by FastQC (version 0.11.5)