FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1032536
Sequences flagged as poor quality0
Sequence length35-251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGA29050.098.3551949
GGAGGCT24450.086.2654959
TACTCGG6600.083.6054843
GCTACTC16800.077.5229341
GTGCTGG22900.077.128547
AGTAGCT15000.076.842735
AAGTGCT25250.076.729415
TGCTGGG25750.076.195668
AGTGCTG25100.074.3049556
AAAGTGC25350.074.008164
GAGTAGC11100.070.700614
TCGGGAG8100.070.143496
GTAGCTG15200.069.882666
CAAAGTG28700.069.64213
CTCGGGA7750.069.6172945
CCGAGTA6650.069.553292
CCCAAAG33850.068.3204351
TAGCTGG16150.067.30157
CCAAAGT30950.067.2498252
TTGGGAG16350.067.2336656
Produced by FastQC (version 0.11.5)