FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541240
Sequences flagged as poor quality0
Sequence length35-351
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10900.075.22063
GCTGGGA92600.070.125739
CCGAGTA11900.066.1435552
TACTCGG10600.063.426573
GTAGCTG44700.059.062476
AGTAGCT42750.058.3043175
GAGTAGC26300.057.9856384
CTACTCG11400.057.5371552
TAGCTGG47650.056.782477
GCTACTC28650.056.6635551
CCCAAAG76550.056.5525251
GGAGGCT64850.055.376749
AAAGTGC64850.055.123884
AGTGCTG69500.054.9748886
CCAAAGT72450.054.547132
AAGTGCT69200.053.3174865
GTGCTGG69850.053.0560957
CTCGGGA15500.050.781185
CAAAGTG73850.050.404423
CCCGAGT16700.050.0781
Produced by FastQC (version 0.11.5)