FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541240
Sequences flagged as poor quality0
Sequence length35-251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGA49300.073.9259649
CGAGTAG7200.073.0525053
GGAGGCT35250.065.226849
TAGCTGG25650.063.4060067
GTAGCTG24850.062.9681856
TACTCGG8850.062.1968463
GAGTAGC15100.060.75524
GTGCTGG38400.060.3212747
AGTAGCT26450.060.1196985
CTCGGGA10000.059.937035
CTACTCG10400.059.5193562
AGTGCTG38300.059.1919866
TGCTGGG40050.058.9362378
CCGAGTA8500.058.544532
GCTACTC20550.058.4715731
AAGTGCT37550.058.309895
AAAGTGC37750.058.000964
CCCGAGT9050.057.668851
CAAAGTG45700.052.1936343
ACTCGGG10600.051.92854
Produced by FastQC (version 0.11.5)