FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1471870
Sequences flagged as poor quality0
Sequence length35-351
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA9250.096.999032
CTACTCG10550.094.4961552
CGAGTAG9200.093.914113
TACTCGG11650.091.2786943
GCTGGGA92500.087.119519
GCTACTC29600.078.025841
GGAGGCT63300.077.959339
CCCGAGT13050.077.666671
GTAGCTG41550.077.579346
GAGTAGC23500.076.360944
TAGCTGG44250.075.849637
AGTAGCT42300.075.418215
GTGCTGG70350.070.619157
CTCGGGA14400.069.231565
AGTGCTG74200.068.522456
CCCAAAG78350.068.498161
AAAGTGC69800.068.319054
AAGTGCT72550.067.332585
TGCTGGG78250.066.674648
ACTCGGG15750.066.4622964
Produced by FastQC (version 0.11.5)