FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1534347
Sequences flagged as poor quality0
Sequence length35-251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG7450.060.6689073
CTACTCG8650.060.289162
GCTGGGA63550.058.631879
CCGAGTA7600.055.852342
TACTCGG9600.054.7164573
GCTACTC19950.052.888741
GAGTAGC16050.052.516384
AAAGTGC45000.050.2203754
CCAAAGT50350.050.10152
AGTGCTG45600.049.654586
AAGTGCT46300.048.5464635
CCCAAAG56550.047.825461
CAAAGTG50600.047.8008273
GTAGCTG28700.046.3071636
GTGCTGG50350.045.7033927
AGTAGCT29050.044.9943925
CTCGGGA12250.044.8742455
TCGGGAG12850.044.0515336
GGAGGCT47450.043.511099
ACTCGGG13900.043.062784
Produced by FastQC (version 0.11.5)