FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186821
Sequences flagged as poor quality0
Sequence length35-351
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG7150.055.827873
CTACTCG9850.052.3445362
GCTGGGA69600.052.0924579
CCGAGTA7900.050.527762
TACTCGG9500.047.270053
GGAGGCT49750.044.4617089
CCCAAAG58400.044.143291
GCTACTC21950.043.9480251
AGTAGCT33150.042.646295
TAGCTGG33600.042.5683447
GTAGCTG32600.041.3250436
AAAGTGC49600.040.909494
AGTGCTG50050.040.8739786
GTGCTGG52250.039.7879267
GAGTAGC18800.038.9260834
CCCGAGT10700.038.859941
CAAAGTG54400.038.828513
CCAAAGT57300.038.6049422
AAGTGCT51100.038.40675
TCGGGAG12650.038.128826
Produced by FastQC (version 0.11.5)