FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186821
Sequences flagged as poor quality0
Sequence length35-251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG7200.062.216262
TACTCGG7650.058.99213
GCTGGGA46650.057.7846879
GAGTAGC13450.054.410484
CGAGTAG6250.052.69113
CCGAGTA6800.051.6325722
GCTACTC16500.048.4277881
TCGGGAG10600.046.3634646
CTCGGGA10100.045.8897635
GTAGCTG21700.045.8613666
AGTAGCT22850.045.3718345
ACTCGGG10000.045.1289564
GGAGGCT36050.042.293829
CCCGAGT9900.041.5794141
TAGCTGG25500.041.4362077
AAAGTGC33100.040.902384
AGTGCTG34600.040.8360256
GTGCTGG37150.040.3480647
CCCAAAG44200.039.9912761
CCAAAGT39600.037.910642
Produced by FastQC (version 0.11.5)