FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723813
Sequences flagged as poor quality0
Sequence length35-351
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10450.074.746463
GCTGGGA96050.064.8847969
CCGAGTA12350.064.592692
GAGTAGC26650.054.877524
AGTAGCT45600.053.2104645
AGTGCTG71800.052.0795366
GTGCTGG73350.050.9790157
TAGCTGG50000.050.854637
CCCAAAG81300.050.6955951
GTAGCTG47550.050.3293156
AAAGTGC72050.047.746934
CCAAAGT79250.047.6031462
CCCGAGT17150.047.4833151
CAAAGTG79100.047.273213
TGCTGGG84850.047.2035038
AAGTGCT76450.046.5206035
GGAGGCT68050.045.9133269
TACTCGG13300.044.984193
GCTACTC31550.044.774281
CTACTCG13750.042.3033182
Produced by FastQC (version 0.11.5)