FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487235
Sequences flagged as poor quality0
Sequence length35-351
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA9850.070.2337342
GCTGGGA90450.064.7329
CGAGTAG9400.062.8258443
AGTAGCT39450.060.735065
GTAGCTG40900.057.344226
TAGCTGG41850.057.252047
GAGTAGC24900.056.921734
TACTCGG10800.056.2440873
CCCAAAG70950.055.8873671
AAAGTGC61900.054.7902874
GCTACTC26500.054.121671
CTACTCG10400.053.5400472
AAGTGCT64200.053.0902145
CCCGAGT14200.052.2832371
AGTGCTG66950.051.9176186
CCAAAGT67300.051.39692
GGAGGCT65550.049.9375469
CAAAGTG69300.049.4266243
GTGCTGG72550.047.677627
TGCTGGG81250.045.8951768
Produced by FastQC (version 0.11.5)