FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487235
Sequences flagged as poor quality0
Sequence length35-251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG7650.068.7823
GCTGGGA62900.068.085649
GAGTAGC16800.067.739424
AGTAGCT26400.066.746165
GGAGGCT45300.062.12529
CCGAGTA8350.061.1916922
GTAGCTG27600.060.754816
TACTCGG9550.060.222983
GCTACTC19800.060.2130361
CTACTCG9900.058.98422
TAGCTGG31150.057.3932467
AGTGCTG44550.055.351976
CTCGGGA12500.051.8840185
GTGCTGG48600.051.75417
AAGTGCT43350.051.0925065
TCGGGAG14450.050.342536
CCAAAGT50500.048.9028632
TGCTGGG54950.048.543048
CCCAAAG55750.047.570891
AAAGTGC46200.047.14614
Produced by FastQC (version 0.11.5)