FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951340
Sequences flagged as poor quality0
Sequence length35-351
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG7000.0112.952053
CCGAGTA8200.0102.577122
TACTCGG8350.0100.7344063
GAGTAGC17250.094.5919344
AGTAGCT29500.093.51765
GTAGCTG31150.091.264146
CTACTCG8700.090.880972
GCTGGGA76550.089.877229
TAGCTGG34450.084.9633267
GGAGGCT49950.079.4781959
GCTACTC21400.078.614631
CCCGAGT11000.074.9411851
AAAGTGC49700.074.462534
TCGGGAG13000.073.756956
ACTCGGG12400.073.256064
CCCAAAG60950.071.4896551
AAGTGCT51750.070.8626865
CCAAAGT55500.070.62482
AGTGCTG53850.069.6611566
CAAAGTG55400.069.537663
Produced by FastQC (version 0.11.5)