FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951340
Sequences flagged as poor quality0
Sequence length35-251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG5350.0100.646593
CCGAGTA5650.096.720122
GAGTAGC11650.091.388914
GCTGGGA45550.089.7438359
CTACTCG6950.089.11242
TACTCGG6900.088.679063
GTAGCTG19550.086.3894356
AGTGCTG30050.085.741016
AGTAGCT19450.084.3112265
AAAGTGC30800.083.4389344
GCTACTC15500.081.480641
AAGTGCT31200.080.800275
GGAGGCT38650.078.532389
CAAAGTG34400.076.4856953
TAGCTGG22500.075.062817
GTGCTGG35600.070.642647
CCAAAGT36650.070.576222
CCCAAAG39950.069.306011
CCCGAGT8500.068.576471
CTCGGGA9000.066.627545
Produced by FastQC (version 0.11.5)