FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262187
Sequences flagged as poor quality0
Sequence length35-351
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGA71550.053.871659
CTACTCG8750.053.4001272
TACTCGG9200.052.6020283
GAGTAGC20250.050.2666474
GCTACTC19950.049.3516241
TAGCTGG35650.048.209837
AGTAGCT33800.047.888385
GTAGCTG35250.045.4451186
GGAGGCT50550.045.2227869
CCAAAGT55600.043.81982
CCCAAAG61650.043.0384251
AGTGCTG52550.042.868296
GTGCTGG55350.041.301037
AAGTGCT55000.039.745115
AAAGTGC53600.039.537944
TGCTGGG65350.037.5344168
CGAGTAG8950.037.2905923
ACTCGGG13100.036.940424
CAAAGTG60400.036.7457433
CTCGGGA13450.034.7384875
Produced by FastQC (version 0.11.5)