FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1262187
Sequences flagged as poor quality0
Sequence length35-251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG7300.069.7673952
TACTCGG8350.064.363723
GCTGGGA50900.062.3872159
GAGTAGC12000.059.0366484
TCGGGAG10500.057.4117626
CGAGTAG5050.055.6303023
GTGCTGG38450.055.3533447
GCTACTC16650.055.350991
GGAGGCT36850.055.3501789
AAGTGCT38450.054.639545
AGTGCTG38150.053.853766
AAAGTGC36550.053.8038644
CTCGGGA11250.052.1151165
CCCAAAG45600.050.525991
AGTAGCT22150.050.1813855
CCAAAGT43550.049.8414842
CAAAGTG40850.049.336333
TGCTGGG44450.049.1837128
GTAGCTG22250.048.6571586
ACTCGGG11050.048.6368454
Produced by FastQC (version 0.11.5)