FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250906
Sequences flagged as poor quality0
Sequence length35-351
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG8800.0127.642573
CTACTCG9500.0118.237322
CCGAGTA8250.0113.7987442
GCTGGGA81550.0112.652969
CGAGTAG8250.0109.7345053
GCTACTC25300.0102.714551
AGTAGCT34100.097.341235
GTAGCTG36050.096.72626
TCGGGAG12400.095.989276
GAGTAGC19350.095.301074
GGAGGCT54250.095.1780859
CTCGGGA12500.093.880215
ACTCGGG12400.091.933394
TAGCTGG38750.091.717077
GTGCTGG65150.088.5176857
CCCAAAG74100.088.0140841
AAAGTGC59900.087.879764
TGCTGGG72050.085.624868
AAGTGCT63900.085.264565
CCCGAGT11800.085.249141
Produced by FastQC (version 0.11.5)