FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1250906
Sequences flagged as poor quality0
Sequence length35-251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG9900.0122.049213
GCTACTC22850.0115.0431751
CTACTCG10250.0114.6397552
GGAGGCT51400.0104.944319
GCTGGGA61250.0100.420929
CTCGGGA12700.098.023785
TCGGGAG14000.091.3209466
AGTAGCT24700.090.425135
CGAGTAG6750.090.406823
CCGAGTA6900.089.5382162
GTAGCTG25200.088.784256
ACTCGGG13750.088.763074
GAGTAGC15900.086.739374
TAGCTGG28600.085.10687
GTGCTGG46650.080.373577
AGTGCTG46650.079.846536
TGCTGGG52350.078.562088
AAGTGCT46550.078.394675
AAAGTGC45250.078.219384
CCAAAGT51050.076.884112
Produced by FastQC (version 0.11.5)