FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127501
Sequences flagged as poor quality0
Sequence length35-351
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA9250.0101.903532
CGAGTAG10400.089.016863
AGTAGCT37450.087.6408165
TACTCGG9100.085.086253
TAGCTGG40600.084.5722357
GTAGCTG39000.084.157656
GAGTAGC23100.083.0647054
GCTACTC23550.082.914271
CTACTCG9550.081.076952
GGAGGCT58550.078.767559
GCTGGGA87150.077.060039
CCCGAGT12450.075.7148061
CCCAAAG65500.063.4772071
CCAAAGT60750.060.1251752
CAAAGTG59300.058.4730073
AGTGCTG59600.058.4585086
AAGTGCT59150.058.0495765
AAAGTGC56850.057.7334864
TCGGGAG14400.057.273966
CTCGGGA14450.057.0757835
Produced by FastQC (version 0.11.5)