FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1289161
Sequences flagged as poor quality0
Sequence length35-351
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG9650.057.0034753
GCTGGGA74250.052.9801759
CTACTCG9650.051.821342
AGTAGCT35650.050.030975
GAGTAGC21550.047.9577834
TAGCTGG38750.047.7473347
CGAGTAG9200.047.10863
GTAGCTG36050.046.7014966
GCTACTC23150.046.08511
CTCGGGA13050.044.706665
CCAAAGT57900.043.4723432
CCGAGTA10000.043.3399162
ACTCGGG13550.043.056974
AAAGTGC54900.041.9007154
AAGTGCT56550.041.8572245
CAAAGTG58700.041.7439883
CCCAAAG63900.040.9572071
GGAGGCT57500.040.5842979
AGTGCTG57300.040.4366236
GTGCTGG56250.040.300857
Produced by FastQC (version 0.11.5)