FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1259699
Sequences flagged as poor quality0
Sequence length35-351
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA8900.090.020182
CGAGTAG9150.083.9122543
GAGTAGC23200.065.469954
CCCGAGT13500.064.292181
GCTGGGA86250.058.634739
GTAGCTG40500.058.5223776
AGTAGCT38400.058.2454765
TAGCTGG45100.054.0315867
CCCAAAG65600.049.1068531
CCAAAGT60000.048.9609762
AGTGCTG55900.048.0731666
AAAGTGC53400.047.510654
CTACTCG9550.047.1898962
AAGTGCT56800.046.7237245
TACTCGG10300.045.374253
GTGCTGG60400.044.766127
CAAAGTG62500.043.5307583
GCTACTC23400.041.3714521
GGAGGCT58000.041.150919
TGCTGGG74900.040.5565458
Produced by FastQC (version 0.11.5)