FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434079
Sequences flagged as poor quality0
Sequence length35-251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGA47400.052.0332769
GAGTAGC14950.046.5540164
CGAGTAG5850.041.744343
TAGCTGG25000.041.327677
AGTAGCT21900.041.2582785
AAGTGCT35550.039.8420685
GGAGGCT35950.039.0577359
GTAGCTG23050.038.9541366
AAAGTGC34200.038.915614
AGTGCTG35550.038.7507486
TGCTGGG43000.037.197268
CCGAGTA6550.037.0002372
GCTACTC15750.036.9297641
CCAAAGT41500.036.790722
GTGCTGG38450.036.7877437
CCCGAGT9400.036.0949171
CAAAGTG39750.035.632343
CCCAAAG46700.035.548361
TACTCGG7300.033.4526563
TTGGGAG34450.030.348096
Produced by FastQC (version 0.11.5)