FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200358
Sequences flagged as poor quality0
Sequence length35-351
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGA88450.080.673059
CTACTCG10150.079.398552
CGAGTAG9150.078.901413
TACTCGG10750.076.528823
AGTAGCT37550.076.010995
CCGAGTA9550.075.5966342
GAGTAGC21250.074.268784
GGAGGCT55100.074.044379
TAGCTGG41650.073.7689367
GTAGCTG38800.073.129466
GCTACTC24050.071.9050751
CCCGAGT12450.064.730551
AAAGTGC59250.063.1908044
CCCAAAG69000.063.02141
AAGTGCT61400.062.0718775
ACTCGGG14450.061.580824
AGTGCTG62900.061.3923956
CCAAAGT65650.060.8666842
GTGCTGG64900.060.0178837
CAAAGTG66400.058.9149133
Produced by FastQC (version 0.11.5)