FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200358
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGA41050.086.277829
GTAGCTG19550.079.552396
AGTAGCT19450.077.493135
GGAGGCT29250.077.0914849
TACTCGG7900.076.00583
AGTGCTG28200.073.971946
AAGTGCT28550.073.8244255
AAAGTGC29450.072.400524
TAGCTGG21800.069.64317
GAGTAGC12350.069.455864
TGCTGGG32300.068.6737068
GTGCTGG30100.068.072397
CCAAAGT36400.067.1523362
CCCAAAG39150.067.094741
GCTACTC18650.065.858071
CAAAGTG35750.064.7833563
CTCGGGA8700.063.3827365
ACTCGGG9100.063.289874
TCGGGAG9000.060.344766
CCGAGTA6550.059.4121322
Produced by FastQC (version 0.11.5)