FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences668199
Sequences flagged as poor quality0
Sequence length35-251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGA24350.047.5717059
GTAGCTG10800.046.6154676
TACTCGG3250.045.0080343
TAGCTGG12500.044.2051057
CCGAGTA2752.7284841E-1144.001962
AGTAGCT11100.042.828595
GAGTAGC6100.041.964464
AGTGCTG16250.041.5603566
AAGTGCT18150.039.6248445
GTGCTGG18450.039.2668047
GGAGGCT16900.038.9610949
AAAGTGC17100.037.780874
TTGGGAG17650.036.872376
CGAGTAG3402.5102054E-1035.851993
CAAAGTG19650.035.3593643
CCAAAGT19450.034.2174642
GCTACTC8500.034.1662251
TGCTGGG19350.033.397898
CTACTCG3655.838956E-1033.152162
CTCGGGA4105.2750693E-1132.704015
Produced by FastQC (version 0.11.5)