FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257967
Sequences flagged as poor quality0
Sequence length35-351
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA8050.093.080122
CGAGTAG7900.086.599853
GCTGGGA58100.083.547819
GAGTAGC17900.081.90014
CCCGAGT10800.075.418131
TAGCTGG30800.074.0417
GTAGCTG30250.072.156326
AGTAGCT30750.069.3938755
GGAGGCT40650.067.720659
GCTACTC18450.067.103741
TACTCGG8150.065.9555363
CTCGGGA10000.063.5271765
CCCAAAG56150.062.37611
CTACTCG8100.062.34072
GTGCTGG50300.060.8814247
TCGGGAG11250.060.8123446
AGTGCTG49550.059.830516
TGCTGGG53550.058.0990268
CCAAAGT55950.057.644792
AAAGTGC51500.056.299914
Produced by FastQC (version 0.11.5)