FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919351
Sequences flagged as poor quality0
Sequence length35-351
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA14450.052.4584542
CGAGTAG14700.051.5663073
GAGTAGC35300.049.6270144
AGTAGCT57700.044.0819365
TAGCTGG65950.043.420387
GTAGCTG59300.042.6084866
GCTGGGA128900.041.9480369
CCCGAGT21000.039.307031
TACTCGG15450.037.0698853
CTACTCG15100.035.6979982
GGAGGCT88950.034.2761579
GCTACTC36950.033.281421
CCCAAAG97200.031.0227451
AAAGTGC78250.030.3524884
AAGTGCT83900.030.316185
TCGGGAG22400.030.079566
AGCTGGG103100.030.0620568
CCAAAGT86200.029.8988822
AGTGCTG87200.029.7484076
CAAAGTG88050.028.696753
Produced by FastQC (version 0.11.5)