FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1919351
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA9100.050.6928022
CGAGTAG9900.044.480143
GAGTAGC20950.041.4546434
GGAGGCT58900.040.0790989
GCTGGGA78150.039.753989
AGTAGCT34350.038.814915
GTAGCTG34950.038.7764176
GCTACTC25200.037.5749241
TACTCGG11150.037.299493
TAGCTGG40850.036.7950977
CCAAAGT57100.035.7171862
TTGGGAG56000.035.200936
CCCAAAG67000.034.969271
CTACTCG10250.034.346162
AGTGCTG53250.034.2424056
GTGCTGG57650.033.766037
AAAGTGC51600.033.661814
CAAAGTG58500.033.4551473
AAGTGCT53700.032.573215
ACTCGGG15250.032.0840384
Produced by FastQC (version 0.11.5)