FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853206
Sequences flagged as poor quality0
Sequence length35-351
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGA56300.081.28429
CCGAGTA6550.075.046932
GTAGCTG24600.074.415756
AGTAGCT24600.073.7267155
GAGTAGC15450.071.311754
TAGCTGG26400.069.337897
CGAGTAG6700.068.3073
GCTACTC17500.064.895261
GGAGGCT41350.064.3539059
AAAGTGC40300.064.352084
AGTGCTG39350.064.182676
AAGTGCT40850.063.485655
CCCAAAG44700.063.3264541
CCAAAGT42350.063.238262
TACTCGG6800.059.824433
GTGCTGG43550.058.3786287
CAAAGTG45450.058.1790963
CCCGAGT9500.057.0955961
TGCTGGG51800.054.643378
CTACTCG7400.052.6832242
Produced by FastQC (version 0.11.5)