FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853206
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAGC8600.091.316094
CGAGTAG4150.088.703133
GCTGGGA30550.085.156199
AGTAGCT13500.083.621875
TAGCTGG14750.082.1361167
GTAGCTG14700.079.892586
TACTCGG5400.079.531663
CCGAGTA4450.076.7982
GGAGGCT21850.075.256669
CTCGGGA6250.070.678665
GCTACTC12900.069.06931
AAAGTGC21500.067.345624
TCGGGAG6550.066.058226
AAGTGCT22100.065.517235
AGTGCTG21650.065.0949256
ACTCGGG6750.063.6253364
GTGCTGG22750.061.947487
CCAAAGT24600.060.034672
TGCTGGG25750.059.5695578
CCCGAGT6600.059.177681
Produced by FastQC (version 0.11.5)