FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences982965
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG5650.0103.807673
CTACTCG5650.0100.874022
CGAGTAG5400.090.5113143
GCTACTC15400.088.979031
CCGAGTA6100.081.5049442
GAGTAGC12150.079.448824
GGAGGCT36600.074.111589
GCTGGGA52200.073.9657759
AGTAGCT21000.073.314165
GTAGCTG20600.071.6897056
TCGGGAG9400.070.698266
CCCGAGT8650.068.692591
CTCGGGA8900.068.6462255
ACTCGGG8900.068.6462254
TAGCTGG23650.064.525797
AAGTGCT33400.062.1926545
AAAGTGC33900.060.9149134
AGTGCTG35200.060.8344156
CCCAAAG42350.057.8399471
CCAAAGT38300.057.6238862
Produced by FastQC (version 0.11.5)