FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932118
Sequences flagged as poor quality0
Sequence length35-351
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA7450.097.218842
CGAGTAG7750.089.108763
TACTCGG7650.083.66823
CTACTCG6800.081.741622
GCTGGGA65950.080.706879
GAGTAGC17200.077.363644
AGTAGCT28850.072.395935
CCCGAGT10750.070.508641
TAGCTGG31400.069.735187
GCTACTC16950.069.561121
GTAGCTG31350.068.771846
CCCAAAG52900.065.910241
CCAAAGT50000.064.679922
GTGCTGG49000.063.249927
GGAGGCT45100.062.7438249
AAGTGCT47400.061.8314065
AGTGCTG48350.061.6616366
AAAGTGC46250.061.5478944
CAAAGTG51450.059.255883
TGCTGGG58350.058.310778
Produced by FastQC (version 0.11.5)