FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762266

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762266
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences932118
Sequences flagged as poor quality0
Sequence length35-251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG5200.082.244533
CCGAGTA5500.077.191132
GCTGGGA40400.075.303139
GAGTAGC11850.071.1497044
CCCGAGT6800.067.785491
AGTAGCT19950.067.373975
GGAGGCT29250.065.161919
TAGCTGG21650.064.241997
GTAGCTG20600.063.931526
AAAGTGC26950.062.11614
GCTACTC12200.061.6444471
CCAAAGT32550.061.488662
AAGTGCT28250.060.9878045
AGTGCTG28500.060.461886
GTGCTGG28550.060.3559887
CCCAAAG35700.059.8007351
TACTCGG5350.059.382973
CAAAGTG33200.056.6793673
TGCTGGG34700.054.5754478
TTGGGAG23650.047.880116
Produced by FastQC (version 0.11.5)