FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145277
Sequences flagged as poor quality0
Sequence length35-251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG7950.094.912122
TACTCGG7650.094.422423
GCTACTC17200.094.106651
GCTGGGA54600.077.3384869
GGAGGCT40400.072.407879
GAGTAGC14900.070.663764
TCGGGAG11450.068.926226
ACTCGGG11600.067.547074
AGTAGCT23700.067.155195
CGAGTAG6400.066.95343
GTAGCTG24700.065.900236
CTCGGGA11350.065.798885
AGTGCTG38650.063.172156
TAGCTGG26550.062.701687
AAGTGCT38400.060.576885
GTGCTGG41200.060.1601337
CCAAAGT42050.059.6208762
AAAGTGC37350.059.329754
CCCAAAG46100.058.870951
TGCTGGG45850.058.195098
Produced by FastQC (version 0.11.5)