FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347340
Sequences flagged as poor quality0
Sequence length35-351
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA9750.084.074282
CGAGTAG10250.073.444683
CCCGAGT14850.059.706431
GCTGGGA81550.057.2315869
GAGTAGC23450.054.9292264
GTAGCTG38300.053.2864276
TAGCTGG43350.052.867297
AGTAGCT39000.052.3300065
CCCAAAG66100.048.0865751
CCAAAGT61050.046.309782
TACTCGG8700.046.1491473
GCTACTC23650.045.9784051
CTACTCG9600.045.3078882
AAAGTGC55750.044.109084
AGTGCTG61250.042.879446
AAGTGCT59650.042.3469435
GTGCTGG65250.041.7890627
CAAAGTG63250.040.9961283
GGAGGCT59350.040.306119
CGGAGGT13300.038.991117
Produced by FastQC (version 0.11.5)