FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347340
Sequences flagged as poor quality0
Sequence length35-251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGA59250.059.743399
CGAGTAG6550.059.6310543
AGTAGCT25600.056.260795
CCGAGTA7600.055.8029022
GAGTAGC15600.053.986894
TAGCTGG28500.053.923837
GCTACTC19300.053.3659131
TACTCGG7550.053.3495563
GTAGCTG26550.051.8643656
CTACTCG8450.051.623582
GGAGGCT41600.051.054749
AGTGCTG39350.049.3659936
AAAGTGC39050.049.0729834
CCAAAGT44200.049.0719262
AAGTGCT39900.048.9452935
CCCAAAG49550.047.6862681
CAAAGTG47200.044.2199173
GTGCTGG44250.044.1773227
TGCTGGG50150.043.324078
CCCGAGT10450.040.583931
Produced by FastQC (version 0.11.5)