FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1228061
Sequences flagged as poor quality0
Sequence length35-251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA7200.086.1708152
CGAGTAG7200.085.056793
TACTCGG9400.080.785863
CTACTCG9050.077.965412
GCTGGGA63000.073.492119
GAGTAGC16000.073.4890754
AGTAGCT25700.072.9171755
GTAGCTG27000.072.662256
TAGCTGG29400.071.347227
GGAGGCT47500.067.30339
ACTCGGG12350.065.455864
GCTACTC19550.064.093351
TCGGGAG12650.062.4259156
CTCGGGA12250.060.9909325
AGTGCTG40150.059.9272736
AAGTGCT40850.058.767275
CCCAAAG49900.058.7542151
CCCGAGT11450.058.43591
CCAAAGT46500.058.0794562
AAAGTGC41150.056.552954
Produced by FastQC (version 0.11.5)