FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514582
Sequences flagged as poor quality0
Sequence length35-351
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA9700.095.14622
CGAGTAG10150.085.968193
GCTGGGA88750.075.249029
GAGTAGC23650.068.821924
CTACTCG11550.068.28362
CCCGAGT14900.067.57181
TACTCGG11900.066.275253
GTAGCTG41000.065.4820566
AGTAGCT41000.064.663535
TAGCTGG44300.061.7404867
GGAGGCT63250.061.2826589
GTGCTGG68150.057.122647
AGTGCTG69350.056.376186
GCTACTC29950.056.0278131
AAAGTGC67200.055.9325834
AAGTGCT70600.054.6649975
TGCTGGG79250.052.2978558
CCCAAAG79250.051.6643641
CCAAAGT75500.050.6743772
CAAAGTG75700.049.6538163
Produced by FastQC (version 0.11.5)