FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1709851
Sequences flagged as poor quality0
Sequence length35-351
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG12850.084.3624953
CCGAGTA13550.083.696792
GCTGGGA110050.065.165489
GAGTAGC32900.064.886344
TACTCGG13100.063.6557923
CTACTCG13550.062.77262
CCCGAGT19600.061.2690281
GTAGCTG50350.059.622786
AGTAGCT51600.058.178435
TAGCTGG56200.056.087327
GCTACTC32300.055.2517241
AGTGCTG77900.054.165446
AAAGTGC75500.052.3528824
AAGTGCT79600.051.5419965
CCCAAAG86400.051.349061
CCAAAGT81100.050.383052
GTGCTGG82950.049.4604347
CTCGGGA17550.047.5151445
CAAAGTG85650.047.1223753
GGAGGCT82450.046.5239269
Produced by FastQC (version 0.11.5)