FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408618
Sequences flagged as poor quality0
Sequence length35-351
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG9550.092.8725743
CCGAGTA10350.090.4547962
TACTCGG10350.080.9332353
CTACTCG10100.074.805512
GCTGGGA88850.074.313169
GAGTAGC25000.072.268624
GTAGCTG41050.070.820216
AGTAGCT42250.069.19755
TAGCTGG44850.068.481977
GCTACTC24000.066.383111
ACTCGGG14500.062.300544
GGAGGCT64200.061.1448679
CCCGAGT16250.060.6451
AAAGTGC61100.060.2148934
TCGGGAG15550.060.206256
CTCGGGA14800.059.9279145
AAGTGCT64000.058.769585
AGTGCTG65950.058.0280886
GTGCTGG66050.057.9402287
CCCAAAG77400.055.1733741
Produced by FastQC (version 0.11.5)