FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762280

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762280
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408618
Sequences flagged as poor quality0
Sequence length35-251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGA57600.074.936679
TACTCGG9350.073.051783
GAGTAGC14950.070.9794164
CTACTCG9950.069.36272
GCTACTC20450.068.6813661
CGAGTAG7150.068.235183
AAAGTGC38150.062.98344
GTAGCTG24900.062.6453866
AGTGCTG39600.062.3426936
AGTAGCT23950.062.131055
GGAGGCT43800.060.9654279
AAGTGCT41300.060.5422025
CCGAGTA8050.060.1642272
TAGCTGG27550.059.7403647
GTGCTGG42900.058.9786227
CCCAAAG52200.058.6846161
TCGGGAG10450.056.416956
CCAAAGT48300.055.902592
TGCTGGG49200.055.0175828
CAAAGTG47350.054.094573
Produced by FastQC (version 0.11.5)