FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1438437
Sequences flagged as poor quality0
Sequence length35-251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG8700.085.0833052
TACTCGG10100.076.2694243
TCGGGAG12650.074.961476
GCTGGGA57400.068.798019
CTCGGGA12650.068.627645
GGAGGCT43400.066.7642449
ACTCGGG12300.066.60414
GCTACTC22650.066.433521
AGTGCTG40650.059.9849856
CCGAGTA7100.058.1104662
GTGCTGG42600.058.106457
CGAGTAG6750.057.9665533
AGTAGCT24200.057.599775
AAAGTGC40500.057.3627364
AAGTGCT41850.057.2653435
GTAGCTG24800.057.1063046
TGCTGGG48650.054.2820438
CCAAAGT45800.052.990532
TAGCTGG28600.052.963547
CCCAAAG49000.052.501721
Produced by FastQC (version 0.11.5)