FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1024522
Sequences flagged as poor quality0
Sequence length35-351
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGGA66950.085.3822259
GTAGCTG27600.080.6460656
TACTCGG8850.080.024753
CGAGTAG7100.078.374353
CCGAGTA8150.076.553042
AGTAGCT29250.076.09685
GAGTAGC17400.074.620794
TAGCTGG31100.073.738947
CTACTCG8850.072.403352
CCAAAGT48300.071.918142
CCCAAAG51800.071.61621
AAGTGCT45550.071.077355
AAAGTGC45700.070.8440554
AGTGCTG48150.068.990346
GGAGGCT47050.068.452949
GTGCTGG49700.066.838737
CAAAGTG49900.066.570843
CTCGGGA11400.063.6036345
GCTACTC18850.063.5133971
TGCTGGG56400.063.383348
Produced by FastQC (version 0.11.5)