FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762286

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762286
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1432940
Sequences flagged as poor quality0
Sequence length35-251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG9100.087.259463
CTACTCG9750.085.806622
GGAGGCT45200.081.613689
GCTACTC21500.080.080391
GCTGGGA58900.077.76979
CGAGTAG7450.073.789893
GAGTAGC14950.073.543094
CCGAGTA8150.072.897862
TCGGGAG11550.071.375956
AAGTGCT41550.071.151655
AGTGCTG41200.070.182656
AAAGTGC42250.068.237944
AGTAGCT24500.067.8130655
GTAGCTG25700.065.112586
CTCGGGA12400.065.022385
CAAAGTG47650.063.3249173
GTGCTGG45500.062.738737
TAGCTGG29100.062.5788887
ACTCGGG12650.061.8059164
CCAAAGT50950.060.9217072
Produced by FastQC (version 0.11.5)