FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005762289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005762289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727985
Sequences flagged as poor quality0
Sequence length35-351
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG11850.0100.629263
CCGAGTA13200.095.427082
TACTCGG15450.089.139383
CTACTCG14600.088.577232
AGTAGCT51800.086.245445
TAGCTGG57950.084.627927
GAGTAGC31250.082.766574
GTAGCTG54650.082.055066
GCTGGGA130400.079.725529
GGAGGCT93500.076.1620259
GCTACTC38050.072.830541
CCCGAGT18250.069.941511
CCAAAGT87400.064.567232
ACTCGGG22700.063.629264
AAGTGCT83100.062.855555
CCCAAAG100350.062.26011
AAAGTGC83750.060.7634054
CAAAGTG89650.059.574593
TCGGGAG24150.059.1134156
AGTGCTG91100.058.0732886
Produced by FastQC (version 0.11.5)